diff --git a/.changeset/tender-radios-lose.md b/.changeset/tender-radios-lose.md
new file mode 100644
index 0000000000..84cbaaaa53
--- /dev/null
+++ b/.changeset/tender-radios-lose.md
@@ -0,0 +1,5 @@
+---
+"@lynx-js/react": patch
+---
+
+Fix memory leak by clearing list callbacks when __DestroyLifetime event is triggered.
diff --git a/packages/react/runtime/__test__/list.test.jsx b/packages/react/runtime/__test__/list.test.jsx
index 72e7279599..b08ccbb2cc 100644
--- a/packages/react/runtime/__test__/list.test.jsx
+++ b/packages/react/runtime/__test__/list.test.jsx
@@ -4380,3 +4380,33 @@ describe('update-list-info profile', () => {
     `);
   });
 });
+
+describe('clear __UpdateListCallbacks', () => {
+  it('should register __DestroyLifetime listener and clear callbacks when triggered', () => {
+    const s1 = __SNAPSHOT__(
+      <view>
+        <text>test</text>
+        <list>{HOLE}</list>
+      </view>,
+    );
+
+    const a = new SnapshotInstance(s1);
+    a.ensureElements();
+
+    expect(lynx.getNative().addEventListener).toHaveBeenCalledWith(
+      '__DestroyLifetime',
+      expect.any(Function),
+    );
+
+    const listElement = a.__elements[3];
+    expect(listElement.componentAtIndex).not.toBeNull();
+    expect(listElement.enqueueComponent).not.toBeNull();
+    expect(listElement.componentAtIndexes).not.toBeNull();
+
+    lynx.getNative().dispatchEvent({ type: '__DestroyLifetime', data: {} });
+
+    expect(listElement.componentAtIndex).toBeNull();
+    expect(listElement.enqueueComponent).toBeNull();
+    expect(listElement.componentAtIndexes).toBeNull();
+  });
+});
diff --git a/packages/react/runtime/__test__/utils/globals.js b/packages/react/runtime/__test__/utils/globals.js
index feb13c4c8e..0ca272d334 100644
--- a/packages/react/runtime/__test__/utils/globals.js
+++ b/packages/react/runtime/__test__/utils/globals.js
@@ -13,6 +13,37 @@ const app = {
   }),
 };
 
+const native = {
+  _listeners: {},
+  onTriggerEvent: undefined,
+  postMessage: vi.fn((_message) => {}),
+  addEventListener: vi.fn((type, listener) => {
+    if (!native._listeners[type]) {
+      native._listeners[type] = [];
+    }
+    native._listeners[type].push(listener);
+  }),
+  removeEventListener: vi.fn((type, listener) => {
+    if (native._listeners[type]) {
+      native._listeners[type] = native._listeners[type].filter(l => l !== listener);
+    }
+  }),
+  dispatchEvent: vi.fn((event) => {
+    if (native._listeners[event.type]) {
+      native._listeners[event.type].forEach(listener => listener(event));
+    }
+    return { canceled: false };
+  }),
+  _clear: () => {
+    native._listeners = {};
+    native.onTriggerEvent = undefined;
+    native.postMessage.mockClear();
+    native.addEventListener.mockClear();
+    native.removeEventListener.mockClear();
+    native.dispatchEvent.mockClear();
+  },
+};
+
 const performance = {
   __functionCallHistory: [],
   _generatePipelineOptions: vi.fn(() => {
@@ -126,6 +157,7 @@ function injectGlobals() {
   globalThis.lynx = {
     queueMicrotask: Promise.prototype.then.bind(Promise.resolve()),
     getNativeApp: () => app,
+    getNative: () => native,
     performance,
     createSelectorQuery: () => {
       return new SelectorQuery();
@@ -169,6 +201,7 @@ function injectGlobals() {
 beforeEach(() => {
   performance.profileStart.mockClear();
   performance.profileEnd.mockClear();
+  native._clear();
 });
 
 afterEach((context) => {
diff --git a/packages/react/runtime/src/snapshot/list.ts b/packages/react/runtime/src/snapshot/list.ts
index d7ff523947..35b5e65f25 100644
--- a/packages/react/runtime/src/snapshot/list.ts
+++ b/packages/react/runtime/src/snapshot/list.ts
@@ -22,6 +22,13 @@ export function snapshotCreateList(
     componentAtIndexes,
   );
   const listID = __GetElementUniqueID(list);
+
+  if (typeof lynx !== 'undefined' && typeof lynx.getNative === 'function') {
+    lynx.getNative()?.addEventListener('__DestroyLifetime', () => {
+      __UpdateListCallbacks(list, null, null, null);
+    });
+  }
+
   gSignMap[listID] = signMap;
   gRecycleMap[listID] = recycleMap;
   return list;
diff --git a/packages/react/runtime/types/types.d.ts b/packages/react/runtime/types/types.d.ts
index 7364370a38..42828b421f 100644
--- a/packages/react/runtime/types/types.d.ts
+++ b/packages/react/runtime/types/types.d.ts
@@ -98,9 +98,9 @@ declare global {
   ): void;
   declare function __UpdateListCallbacks(
     list: FiberElement,
-    componentAtIndex: ComponentAtIndexCallback,
-    enqueueComponent: EnqueueComponentCallback,
-    componentAtIndexes: ComponentAtIndexesCallback,
+    componentAtIndex: ComponentAtIndexCallback | null,
+    enqueueComponent: EnqueueComponentCallback | null,
+    componentAtIndexes: ComponentAtIndexesCallback | null,
   ): void;
   declare function __OnLifecycleEvent(...args: any[]): void;
   declare function _ReportError(
diff --git a/packages/testing-library/testing-environment/src/index.ts b/packages/testing-library/testing-environment/src/index.ts
index 601e7ea3ff..73b2f52bc1 100644
--- a/packages/testing-library/testing-environment/src/index.ts
+++ b/packages/testing-library/testing-environment/src/index.ts
@@ -249,8 +249,36 @@ function injectMainThreadGlobals(target?: any, polyfills?: any) {
   target.__TESTING_FORCE_RENDER_TO_OPCODE__ = false;
   target.__ENABLE_SSR__ = false;
   target.globDynamicComponentEntry = '__Card__';
+
+  const native = {
+    _listeners: {} as Record<string, ((event: any) => void)[]>,
+    onTriggerEvent: undefined as ((event: any) => void) | undefined,
+    postMessage: ((_message: any) => {}),
+    addEventListener: ((type: string, listener: (event: any) => void) => {
+      if (!native._listeners[type]) {
+        native._listeners[type] = [];
+      }
+      native._listeners[type].push(listener);
+    }),
+    removeEventListener: ((type: string, listener: (event: any) => void) => {
+      if (native._listeners[type]) {
+        native._listeners[type] = native._listeners[type].filter(l =>
+          l !== listener
+        );
+      }
+    }),
+    dispatchEvent: ((event: { type: string; data?: any }) => {
+      const listeners = native._listeners[event.type];
+      if (listeners) {
+        listeners.forEach(listener => listener(event));
+      }
+      return { canceled: false };
+    }),
+  };
+
   target.lynx = {
     performance,
+    getNative: () => native,
     getCoreContext: (() => CoreContext),
     /*