diff --git a/.changeset/inline-worklet-runtime.md b/.changeset/inline-worklet-runtime.md
new file mode 100644
index 0000000000..05e2dea53e
--- /dev/null
+++ b/.changeset/inline-worklet-runtime.md
@@ -0,0 +1,6 @@
+---
+"@lynx-js/react-rsbuild-plugin": minor
+"@lynx-js/react-webpack-plugin": minor
+---
+
+Inline worklet-runtime into main-thread.js entry instead of injecting it as a separate Lepus chunk.
diff --git a/packages/react/worklet-runtime/src/bindings/loadRuntime.ts b/packages/react/worklet-runtime/src/bindings/loadRuntime.ts
index 325e6618c4..294ff3927f 100644
--- a/packages/react/worklet-runtime/src/bindings/loadRuntime.ts
+++ b/packages/react/worklet-runtime/src/bindings/loadRuntime.ts
@@ -5,21 +5,18 @@
 import '../global.js';
 
 /**
- * Loads and initializes the Lepus chunk in the main thread.
- * @param __schema - The dynamic component entry for loading the Lepus chunk.
- * @returns A boolean indicating whether the Lepus chunk was loaded and initialized successfully.
+ * Guard function for worklet runtime availability.
+ *
+ * The worklet-runtime is now bundled directly into the main-thread.js entry,
+ * so there is no need to load it via `__LoadLepusChunk`. This function is
+ * kept as a guard because SWC-generated code still calls it before
+ * `registerWorkletInternal`.
+ *
+ * @param __schema - Unused. Kept for backward compatibility with SWC-generated call sites.
+ * @returns Whether the worklet runtime has been initialized.
  */
 function loadWorkletRuntime(__schema?: string): boolean {
-  if (typeof __LoadLepusChunk === 'undefined') {
-    return false;
-  }
-  if (globalThis.lynxWorkletImpl) {
-    return true;
-  }
-  return __LoadLepusChunk('worklet-runtime', {
-    dynamicComponentEntry: __schema,
-    chunkType: 0,
-  });
+  return !!globalThis.lynxWorkletImpl;
 }
 
 export { loadWorkletRuntime };
diff --git a/packages/rspeedy/plugin-react/src/entry.ts b/packages/rspeedy/plugin-react/src/entry.ts
index 52151fa067..89aa281543 100644
--- a/packages/rspeedy/plugin-react/src/entry.ts
+++ b/packages/rspeedy/plugin-react/src/entry.ts
@@ -63,6 +63,14 @@ export function applyEntry(
     const enableChunkSplitting =
       rsbuildConfig.performance?.chunkSplit?.strategy !== 'all-in-one'
 
+    const { resolve } = api.useExposed<
+      { resolve: (request: string) => Promise<string> }
+    >(Symbol.for('@lynx-js/react/internal:resolve'))!
+
+    const workletRuntimePath = await resolve(
+      `@lynx-js/react/${isDev ? 'worklet-dev-runtime' : 'worklet-runtime'}`,
+    )
+
     const isRspeedy = api.context.callerName === 'rspeedy'
     if (isRspeedy) {
       // biome-ignore lint/correctness/useHookAtTopLevel: This is not a React hook.
@@ -127,7 +135,7 @@ export function applyEntry(
           .entry(mainThreadEntry)
           .add({
             layer: LAYERS.MAIN_THREAD,
-            import: imports,
+            import: [...imports, workletRuntimePath],
             filename: mainThreadName,
           })
           .when(enabledHMR, entry => {
@@ -261,10 +269,6 @@ export function applyEntry(
       extractStr = false
     }
 
-    const { resolve } = api.useExposed<
-      { resolve: (request: string) => Promise<string> }
-    >(Symbol.for('@lynx-js/react/internal:resolve'))!
-
     chain
       .plugin(PLUGIN_NAME_REACT)
       .after(PLUGIN_NAME_TEMPLATE)
@@ -277,9 +281,7 @@ export function applyEntry(
         extractStr,
         experimental_isLazyBundle,
         profile: getDefaultProfile(),
-        workletRuntimePath: await resolve(
-          `@lynx-js/react/${isDev ? 'worklet-dev-runtime' : 'worklet-runtime'}`,
-        ),
+        workletRuntimePath,
       }])
 
     function getDefaultProfile(): boolean | undefined {
diff --git a/packages/webpack/react-webpack-plugin/src/ReactWebpackPlugin.ts b/packages/webpack/react-webpack-plugin/src/ReactWebpackPlugin.ts
index af12ce91f5..075f535764 100644
--- a/packages/webpack/react-webpack-plugin/src/ReactWebpackPlugin.ts
+++ b/packages/webpack/react-webpack-plugin/src/ReactWebpackPlugin.ts
@@ -2,7 +2,6 @@
 // Licensed under the Apache License Version 2.0 that can be found in the
 // LICENSE file in the root directory of this source tree.
 
-import * as fs from 'node:fs';
 import { createRequire } from 'node:module';
 
 import type { Chunk, Compilation, Compiler } from '@rspack/core';
@@ -262,30 +261,7 @@ class ReactWebpackPlugin {
       // @ts-expect-error Rspack x Webpack compilation not match
       const hooks = LynxTemplatePlugin.getLynxTemplatePluginHooks(compilation);
 
-      const { RawSource, ConcatSource } = compiler.webpack.sources;
-      hooks.beforeEncode.tap(
-        this.constructor.name,
-        (args) => {
-          const lepusCode = args.encodeData.lepusCode;
-          if (
-            lepusCode.root?.source.source().toString()?.includes(
-              'registerWorkletInternal',
-            )
-          ) {
-            lepusCode.chunks.push({
-              name: 'worklet-runtime',
-              source: new RawSource(fs.readFileSync(
-                options.workletRuntimePath,
-                'utf8',
-              )),
-              info: {
-                ['lynx:main-thread']: true,
-              },
-            });
-          }
-          return args;
-        },
-      );
+      const { ConcatSource } = compiler.webpack.sources;
 
       // Inject `module.exports` for async main-thread chunks
       hooks.beforeEncode.tap(this.constructor.name, (args) => {
diff --git a/packages/webpack/react-webpack-plugin/test/cases/worklet-runtime/chunk/index.js b/packages/webpack/react-webpack-plugin/test/cases/worklet-runtime/chunk/index.js
index f2ee0341b2..4eab86b3ba 100644
--- a/packages/webpack/react-webpack-plugin/test/cases/worklet-runtime/chunk/index.js
+++ b/packages/webpack/react-webpack-plugin/test/cases/worklet-runtime/chunk/index.js
@@ -6,7 +6,7 @@ import path from 'node:path';
 
 import './a.jsx';
 
-it('should have worklet-runtime', async () => {
+it('should have worklet-runtime inlined in main-thread', async () => {
   const source = await fs.readFile(
     path.resolve(
       path.join(
@@ -18,6 +18,11 @@ it('should have worklet-runtime', async () => {
     'utf-8',
   );
   const json = JSON.parse(source);
-  expect(json['lepusCode']['lepusChunk']['worklet-runtime'].length > 0)
+  // worklet-runtime is now bundled into main-thread.js entry,
+  // not as a separate lepus chunk
+  expect(json['lepusCode']['lepusChunk']['worklet-runtime'])
+    .toBe(undefined);
+  // Verify the worklet runtime code is in the main-thread root
+  expect(json['lepusCode']['root'].includes('lynxWorkletImpl'))
     .toBe(true);
 });
diff --git a/packages/webpack/react-webpack-plugin/test/cases/worklet-runtime/chunk/rspack.config.js b/packages/webpack/react-webpack-plugin/test/cases/worklet-runtime/chunk/rspack.config.js
index 6cebcf7671..3c869d6859 100644
--- a/packages/webpack/react-webpack-plugin/test/cases/worklet-runtime/chunk/rspack.config.js
+++ b/packages/webpack/react-webpack-plugin/test/cases/worklet-runtime/chunk/rspack.config.js
@@ -1,3 +1,4 @@
+import { createRequire } from 'node:module';
 import path from 'node:path';
 import { fileURLToPath } from 'node:url';
 
@@ -8,11 +9,18 @@ import {
 } from '@lynx-js/template-webpack-plugin';
 
 const __dirname = path.dirname(fileURLToPath(import.meta.url));
+const require = createRequire(import.meta.url);
 
 const defaultConfig = createConfig({}, {
   mainThreadChunks: ['main__main-thread.js'],
 }, {});
 
+// Add worklet-runtime to main-thread entry (simulating rspeedy entry setup)
+defaultConfig.entry['main__main-thread'].import = [
+  defaultConfig.entry['main__main-thread'].import,
+  require.resolve('@lynx-js/react/worklet-dev-runtime'),
+].flat();
+
 /** @type {import('@rspack/core').Configuration} */
 export default {
   context: __dirname,