diff --git a/packages/vite/src/node/__tests__/plugins/workerImportMetaUrl.spec.ts b/packages/vite/src/node/__tests__/plugins/workerImportMetaUrl.spec.ts
index 348e3e39be0147..517947b1800667 100644
--- a/packages/vite/src/node/__tests__/plugins/workerImportMetaUrl.spec.ts
+++ b/packages/vite/src/node/__tests__/plugins/workerImportMetaUrl.spec.ts
@@ -253,4 +253,14 @@ new Worker(
       )"
     `)
   })
+
+  test('preserves custom search params in worker URL', async () => {
+    expect(
+      await transform(
+        'new Worker(new URL("./worker.js?foo=bar&baz=qux", import.meta.url), { type: "module" })',
+      ),
+    ).toMatchInlineSnapshot(
+      '"new Worker(new URL(/* @vite-ignore */ "/worker.js?worker_file&type=module&foo=bar&baz=qux", \'\' + import.meta.url), { type: "module" })"',
+    )
+  })
 })
diff --git a/packages/vite/src/node/plugins/worker.ts b/packages/vite/src/node/plugins/worker.ts
index b868df50d392f9..c974817ac1269d 100644
--- a/packages/vite/src/node/plugins/worker.ts
+++ b/packages/vite/src/node/plugins/worker.ts
@@ -23,7 +23,7 @@ import {
   onRollupLog,
   toOutputFilePathInJS,
 } from '../build'
-import { cleanUrl } from '../../shared/utils'
+import { cleanUrl, splitFileAndPostfix } from '../../shared/utils'
 import type { Logger } from '../logger'
 import { fileToUrl, toOutputFilePathInJSForBundledDev } from './asset'
 
@@ -156,6 +156,23 @@ export const workerOrSharedWorkerRE: RegExp =
 const workerFileRE = /(?:\?|&)worker_file&type=(\w+)(?:&|$)/
 const inlineRE = /[?&]inline\b/
 
+function getWorkerRequestPostfix(id: string): string {
+  const { postfix } = splitFileAndPostfix(id)
+  if (!postfix || postfix[0] !== '?') {
+    return ''
+  }
+
+  const queryParams = new URLSearchParams(postfix.slice(1))
+
+  queryParams.delete('worker')
+  queryParams.delete('sharedworker')
+  queryParams.delete('inline')
+  queryParams.delete('url')
+
+  const customQuery = queryParams.toString()
+  return customQuery ? `?${customQuery}` : ''
+}
+
 export const WORKER_FILE_ID = 'worker_file'
 const workerOutputCaches = new WeakMap<ResolvedConfig, WorkerOutputCache>()
 
@@ -489,8 +506,12 @@ export function webWorkerPlugin(config: ResolvedConfig): Plugin {
             }
           }
         } else {
+          const workerRequestPostfix = getWorkerRequestPostfix(id)
           let url = await fileToUrl(this, cleanUrl(id))
-          url = injectQuery(url, `${WORKER_FILE_ID}&type=${workerType}`)
+          url = injectQuery(
+            `${url}${workerRequestPostfix}`,
+            `${WORKER_FILE_ID}&type=${workerType}`,
+          )
           urlCode = JSON.stringify(url)
         }
 
diff --git a/packages/vite/src/node/plugins/workerImportMetaUrl.ts b/packages/vite/src/node/plugins/workerImportMetaUrl.ts
index 55d28d17e0f97b..48150dcc4ea1e6 100644
--- a/packages/vite/src/node/plugins/workerImportMetaUrl.ts
+++ b/packages/vite/src/node/plugins/workerImportMetaUrl.ts
@@ -9,7 +9,7 @@ import type { Plugin } from '../plugin'
 import { evalValue, injectQuery, transformStableResult } from '../utils'
 import { createBackCompatIdResolver } from '../idResolver'
 import type { ResolveIdFn } from '../idResolver'
-import { cleanUrl, slash } from '../../shared/utils'
+import { cleanUrl, slash, splitFileAndPostfix } from '../../shared/utils'
 import type { WorkerType } from './worker'
 import { WORKER_FILE_ID, workerFileToUrl } from './worker'
 import { fileToUrl, toOutputFilePathInJSForBundledDev } from './asset'
@@ -229,9 +229,11 @@ export function workerImportMetaUrlPlugin(config: ResolvedConfig): Plugin {
           s ||= new MagicString(code)
           const workerType = await getWorkerType(code, cleanString, endIndex)
           const url = rawUrl.slice(1, -1)
+          const { file: urlWithoutPostfix, postfix } = splitFileAndPostfix(url)
+          const queryPostfix = postfix[0] === '?' ? postfix : ''
           let file: string | undefined
-          if (url[0] === '.') {
-            file = path.resolve(path.dirname(id), url)
+          if (urlWithoutPostfix[0] === '.') {
+            file = path.resolve(path.dirname(id), urlWithoutPostfix)
             file = slash(tryFsResolve(file, fsResolveOptions) ?? file)
           } else {
             workerResolver ??= createBackCompatIdResolver(config, {
@@ -239,11 +241,11 @@ export function workerImportMetaUrlPlugin(config: ResolvedConfig): Plugin {
               tryIndex: false,
               preferRelative: true,
             })
-            file = await workerResolver(this.environment, url, id)
+            file = await workerResolver(this.environment, urlWithoutPostfix, id)
             file ??=
-              url[0] === '/'
-                ? slash(path.join(config.publicDir, url))
-                : slash(path.resolve(path.dirname(id), url))
+              urlWithoutPostfix[0] === '/'
+                ? slash(path.join(config.publicDir, urlWithoutPostfix))
+                : slash(path.resolve(path.dirname(id), urlWithoutPostfix))
           }
 
           if (
@@ -273,7 +275,7 @@ export function workerImportMetaUrlPlugin(config: ResolvedConfig): Plugin {
             } else {
               builtUrl = await fileToUrl(this, cleanUrl(file))
               builtUrl = injectQuery(
-                builtUrl,
+                `${builtUrl}${queryPostfix}`,
                 `${WORKER_FILE_ID}&type=${workerType}`,
               )
             }